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Titration of DnaA protein by oriC DnaA-boxes increases dnaA gene expression in Escherichia coli.
Binding of the DnaA protein to its binding sites, the DnaA-boxes (TTATCCACA), was measured by a simple physiological approach. The presence of extra DnaA-boxes in growing cells leads to a derepression of dnaA gene expression, measured as beta-galactosidase activity of a dnaA-lacZ fusion polypeptide....
Enregistré dans:
| Publié dans: | EMBO J |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Nature Publishing Group
1987
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC553385/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3034578/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1987.tb04747.x |
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