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Translational roles of the C75 2′OH in an in vitro tRNA transcript at the ribosomal A, P and E sites
Aminoacyl-tRNAs containing a deoxy substitution in the penultimate nucleotide (C75 2′OH → 2′H) have been widely used in translation for incorporation of unnatural amino acids (AAs). However, this supposedly innocuous modification surprisingly increased peptidyl-tRNA(Ala) (ugc) drop off in biochemica...
Shranjeno v:
| izdano v: | Sci Rep |
|---|---|
| Main Authors: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Nature Publishing Group UK
2017
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5532260/ https://ncbi.nlm.nih.gov/pubmed/28751745 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-06991-6 |
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