Isolation and characterization of the retinal-binding component of halorhodopsin

Halorhodopsin (HR) was reconstituted in cell vesicles prepared from Halobacterium halobium strain L-07 by addition of tritium-labelled retinal and subsequently reduced with cyanoborohydride. Lysis of the labelled vesicles in water and dissolution of the cell membranes with 4% SDS allowed the purific...

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Publié dans:	EMBO J
Auteurs principaux:	Hegemann, P., Steiner, M., Oesterhelt, D.
Format:	Artigo
Langue:	Inglês
Publié:	Nature Publishing Group 1982
Sujets:	Articles
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC553186/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16453434/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1982.tb00010.x
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