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Isolation and characterization of the retinal-binding component of halorhodopsin

Halorhodopsin (HR) was reconstituted in cell vesicles prepared from Halobacterium halobium strain L-07 by addition of tritium-labelled retinal and subsequently reduced with cyanoborohydride. Lysis of the labelled vesicles in water and dissolution of the cell membranes with 4% SDS allowed the purific...

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Autori principali: Hegemann, P., Steiner, M., Oesterhelt, D.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1982
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC553186/
https://ncbi.nlm.nih.gov/pubmed/16453434
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