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Gene transfer into mouse lyoma cells by electroporation in high electric fields.

Electric impulses (8 kV/cm, 5 microseconds) were found to increase greatly the uptake of DNA into cells. When linear or circular plasmid DNA containing the herpes simplex thymidine kinase (TK) gene is added to a suspension of mouse L cells deficient in the TK gene and the cells are then exposed to e...

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Detalhes bibliográficos
Publicado no:EMBO J
Principais autores: Neumann, E, Schaefer-Ridder, M, Wang, Y, Hofschneider, P H
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC553119/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6329708/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1982.tb01257.x
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