Gene transfer into mouse lyoma cells by electroporation in high electric fields.

Electric impulses (8 kV/cm, 5 microseconds) were found to increase greatly the uptake of DNA into cells. When linear or circular plasmid DNA containing the herpes simplex thymidine kinase (TK) gene is added to a suspension of mouse L cells deficient in the TK gene and the cells are then exposed to e...

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Bibliografiske detaljer
Main Authors: Neumann, E, Schaefer-Ridder, M, Wang, Y, Hofschneider, P H
Format: Artigo
Sprog:Inglês
Udgivet: 1982
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC553119/
https://ncbi.nlm.nih.gov/pubmed/6329708
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