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Dual functional Phi29 DNA polymerase-triggered exponential rolling circle amplification for sequence-specific detection of target DNA embedded in long-stranded genomic DNA

An exonucleolytic digestion-assisted exponential rolling circle amplification (RCA) strategy was developed for sensitive and sequence-specific detection of target DNA embedded in long-stranded genomic DNA. Herein, Phi29 DNA polymerase plays two important roles as exonuclease and polymerase. Long-str...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Li, Xiao-Yu, Du, Yi-Chen, Zhang, Yu-Peng, Kong, De-Ming
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5524717/
https://ncbi.nlm.nih.gov/pubmed/28740223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-06594-1
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