Rapid, Automated, and Specific Immunoassay to Directly Measure Matrix Metalloproteinase-9–Tissue Inhibitor of Metalloproteinase-1 Interactions in Human Plasma Using AlphaLISA Technology: A New Alternative to Classical ELISA

The protocol describes a novel, rapid, and no-wash one-step immunoassay for highly sensitive and direct detection of the complexes between matrix metalloproteinases (MMPs) and their tissue inhibitor of metalloproteinases (TIMPs) based on AlphaLISA(®) technology. We describe two procedures: (i) one a...

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Detalhes bibliográficos
Publicado no:Front Immunol
Main Authors: Pulido-Olmo, Helena, Rodríguez-Sánchez, Elena, Navarro-García, José Alberto, Barderas, María G., Álvarez-Llamas, Gloria, Segura, Julián, Fernández-Alfonso, Marisol, Ruilope, Luis M., Ruiz-Hurtado, Gema
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2017
Assuntos:
Immunology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5523156/
https://ncbi.nlm.nih.gov/pubmed/28791014
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fimmu.2017.00853
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