Cloning and expression in Escherichia coli of a gene for an alkylbase DNA glycosylase from Saccharomyces cerevisiae; a homologue to the bacterial alkA gene.

An alkylation repair deficient mutant of Escherichia coli (tag ada), lacking DNA glycosylase activity for removal of alkylated bases, was transformed by a genomic yeast DNA library and clones selected which survived plating on medium containing the alkylating agent methylmethane sulphonate. Three di...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Berdal, K G, Bjørås, M, Bjelland, S, Seeberg, E
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1990
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC552254/
https://ncbi.nlm.nih.gov/pubmed/2265619
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