Cloning and expression in Escherichia coli of a gene for an alkylbase DNA glycosylase from Saccharomyces cerevisiae; a homologue to the bacterial alkA gene.

An alkylation repair deficient mutant of Escherichia coli (tag ada), lacking DNA glycosylase activity for removal of alkylated bases, was transformed by a genomic yeast DNA library and clones selected which survived plating on medium containing the alkylating agent methylmethane sulphonate. Three di...

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Enregistré dans:
Détails bibliographiques
Publié dans:EMBO J
Auteurs principaux: Berdal, K G, Bjørås, M, Bjelland, S, Seeberg, E
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group 1990
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC552254/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2265619/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1990.tb07909.x
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