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High-fidelity de novo synthesis of pathways using microchip-synthesized oligonucleotides and general molecular biology equipment

Engineering and evaluation of synthetic routes for generating valuable compounds require accurate and cost-effective de novo synthesis of genetic pathways. Here, we present an economical and streamlined de novo DNA synthesis approach for engineering a synthetic pathway with microchip-synthesized oli...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Wan, Wen, Lu, Min, Wang, Dongmei, Gao, Xiaolian, Hong, Jiong
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5522410/
https://ncbi.nlm.nih.gov/pubmed/28733633
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-06428-0
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