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An in vitro system for the leader-primed transcription of coronavirus mRNAs.
We have developed an in vitro transcription system which can utilize exogenous leader RNA for mouse hepatitis virus (MHV) 'leader-primed' mRNA transcription. Cytoplasmic extracts containing viral proteins and template RNA were prepared by lysolecithin permeabilization of MHV-infected cells...
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| Publicado en: | EMBO J |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Nature Publishing Group
1990
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC552193/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2174358/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1990.tb07641.x |
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