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An in vitro system for the leader-primed transcription of coronavirus mRNAs.

We have developed an in vitro transcription system which can utilize exogenous leader RNA for mouse hepatitis virus (MHV) 'leader-primed' mRNA transcription. Cytoplasmic extracts containing viral proteins and template RNA were prepared by lysolecithin permeabilization of MHV-infected cells...

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Detalhes bibliográficos
Publicado no:EMBO J
Main Authors: Baker, S C, Lai, M M
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 1990
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC552193/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2174358/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1990.tb07641.x
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