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Production, characterization, and assessment of a stable analog of the response regulator CheY‐phosphate from Thermotoga maritima

Phosphorylation of CheY promotes association with the flagellar motor and ultimately controls the directional bias of the motor. However, biochemical studies of activated CheY‐phosphate have been challenging due to the rapid hydrolysis of the aspartyl‐phosphate in vitro. An inert analog of Tm CheY‐p...

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書目詳細資料
發表在:Protein Sci
Main Authors: Beyersdorf, Matthew S., Sircar, Ria, Lookadoo, Daniel B., Bottone, Cory J., Lynch, Michael J., Crane, Brian R., Halkides, Christopher J.
格式: Artigo
語言:Inglês
出版: John Wiley and Sons Inc. 2017
主題:
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5521581/
https://ncbi.nlm.nih.gov/pubmed/28440031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3180
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