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Production, characterization, and assessment of a stable analog of the response regulator CheY‐phosphate from Thermotoga maritima

Phosphorylation of CheY promotes association with the flagellar motor and ultimately controls the directional bias of the motor. However, biochemical studies of activated CheY‐phosphate have been challenging due to the rapid hydrolysis of the aspartyl‐phosphate in vitro. An inert analog of Tm CheY‐p...

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Detalles Bibliográficos
Publicado en:Protein Sci
Main Authors: Beyersdorf, Matthew S., Sircar, Ria, Lookadoo, Daniel B., Bottone, Cory J., Lynch, Michael J., Crane, Brian R., Halkides, Christopher J.
Formato: Artigo
Idioma:Inglês
Publicado: John Wiley and Sons Inc. 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5521581/
https://ncbi.nlm.nih.gov/pubmed/28440031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3180
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