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Production, characterization, and assessment of a stable analog of the response regulator CheY‐phosphate from Thermotoga maritima

Phosphorylation of CheY promotes association with the flagellar motor and ultimately controls the directional bias of the motor. However, biochemical studies of activated CheY‐phosphate have been challenging due to the rapid hydrolysis of the aspartyl‐phosphate in vitro. An inert analog of Tm CheY‐p...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Protein Sci
Hauptverfasser: Beyersdorf, Matthew S., Sircar, Ria, Lookadoo, Daniel B., Bottone, Cory J., Lynch, Michael J., Crane, Brian R., Halkides, Christopher J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: John Wiley and Sons Inc. 2017
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5521581/
https://ncbi.nlm.nih.gov/pubmed/28440031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3180
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