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Resolution of synthetic Holliday junctions in DNA by an endonuclease activity from calf thymus.

Extracts of calf thymus have been fractionated to reveal a nuclease activity that specifically cleaves model Holliday junctions in vitro. The products of cleavage are unbranched linear duplex DNA molecules. Using synthetic four-way junctions, we show that the major sites of cutting are diametrically...

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Detalhes bibliográficos
Publicado no:EMBO J
Main Authors: Elborough, K M, West, S C
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 1990
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC552009/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2167838/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1990.tb07484.x
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