Nucleosome assembly in mammalian cell extracts before and after DNA replication.

Protein-free DNA in a cytosolic extract supplemented with SV40 large T-antigen (T-Ag), is assembled into chromatin structure when nuclear extract is added. This assembly was monitored by topoisomer formation, micrococcal nuclease digestion and psoralen crosslinking of the DNA. Plasmids containing SV...

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Autors principals: Gruss, C, Gutierrez, C, Burhans, W C, DePamphilis, M L, Koller, T, Sogo, J M
Format: Artigo
Idioma:Inglês
Publicat: 1990
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC552007/
https://ncbi.nlm.nih.gov/pubmed/2167837
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