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Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data

Normalization of high-throughput small RNA sequencing (sRNA-Seq) data is required to compare sRNA levels across different samples. Commonly used relative normalization approaches can cause erroneous conclusions due to fluctuating small RNA populations between tissues. We developed a set of sRNA spik...

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Veröffentlicht in:Sci Rep
Hauptverfasser: Lutzmayer, Stefan, Enugutti, Balaji, Nodine, Michael D.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2017
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5517642/
https://ncbi.nlm.nih.gov/pubmed/28724941
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-06174-3
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