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Unzipping single DNA molecules to study nucleosome structure and dynamics
DNA-unzipping is a powerful tool to study protein-DNA interactions at the single-molecule level. In this chapter, we provide a detailed and practical guide to performing this technique with an optical trap, using nucleosome studies as an example. We detail protocols for preparing an unzipping templa...
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| Publié dans: | Methods Enzymol |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2012
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5515249/ https://ncbi.nlm.nih.gov/pubmed/22929764 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-391938-0.00002-1 |
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