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Unzipping single DNA molecules to study nucleosome structure and dynamics

DNA-unzipping is a powerful tool to study protein-DNA interactions at the single-molecule level. In this chapter, we provide a detailed and practical guide to performing this technique with an optical trap, using nucleosome studies as an example. We detail protocols for preparing an unzipping templa...

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Sparad:
Bibliografiska uppgifter
I publikationen:Methods Enzymol
Huvudupphovsmän: Li, Ming, Wang, Michelle D.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2012
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC5515249/
https://ncbi.nlm.nih.gov/pubmed/22929764
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-391938-0.00002-1
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