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Probing protein-DNA interactions by unzipping a single DNA double helix.

We present unzipping force analysis of protein association (UFAPA) as a novel and versatile method for detection of the position and dynamic nature of protein-DNA interactions. A single DNA double helix was unzipped in the presence of DNA-binding proteins using a feedback-enhanced optical trap. When...

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Main Authors: Koch, Steven J, Shundrovsky, Alla, Jantzen, Benjamin C, Wang, Michelle D
Format: Artigo
Jezik:Inglês
Izdano: 2002
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1302211/
https://ncbi.nlm.nih.gov/pubmed/12124289
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