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Transposon Mutagenesis of the Zika Virus Genome Highlights Regions Essential for RNA Replication and Restricted for Immune Evasion

The molecular constraints affecting Zika virus (ZIKV) evolution are not well understood. To investigate ZIKV genetic flexibility, we used transposon mutagenesis to add 15-nucleotide insertions throughout the ZIKV MR766 genome and subsequently deep sequenced the viable mutants. Few ZIKV insertion mut...

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Detalhes bibliográficos
Publicado no:J Virol
Main Authors: Fulton, Benjamin O., Sachs, David, Schwarz, Megan C., Palese, Peter, Evans, Matthew J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5512254/
https://ncbi.nlm.nih.gov/pubmed/28515302
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.00698-17
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