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Multiple Ligand-Bound States of a Phosphohexomutase Revealed by Principal Component Analysis of NMR Peak Shifts

Enzymes sample multiple conformations during their catalytic cycles. Chemical shifts from Nuclear Magnetic Resonance (NMR) are hypersensitive to conformational changes and ensembles in solution. Phosphomannomutase/phosphoglucomutase (PMM/PGM) is a ubiquitous four-domain enzyme that catalyzes phospho...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Xu, Jia, Sarma, Akella V. S., Wei, Yirui, Beamer, Lesa J., Van Doren, Steven R.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5509744/
https://ncbi.nlm.nih.gov/pubmed/28706231
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-05557-w
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