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Expression of the archaebacterial bacterio-opsin gene with and without signal sequences in Escherichia coli: the expressed proteins are located in the membrane but bind retinal poorly.

In a further effort to obtain functional expression of the bacterio-opsin gene (bop) in Escherichia coli, the bop gene with E. coli signal sequences as well as the bop gene with the native presequence were expressed in E. coli. The location of the expressed products in the E. coli cell and their pro...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Karnik, S, Doi, T, Molday, R, Khorana, H G
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1990
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC55079/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2247471/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.22.8955
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