Implementation of a loss-of-function system to determine growth and stress-associated mutagenesis in Bacillus subtilis

A forward mutagenesis system based on the acquisition of mutations that inactivate the thymidylate synthase gene (TMS) and confer a trimethoprim resistant (Tmp(r)) phenotype was developed and utilized to study transcription-mediated mutagenesis (TMM). In addition to thyA, Bacillus subtilis possesses...

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Pubblicato in:PLoS One
Autori principali: Villegas-Negrete, Norberto, Robleto, Eduardo A., Obregón-Herrera, Armando, Yasbin, Ronald E., Pedraza-Reyes, Mario
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2017
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5507404/
https://ncbi.nlm.nih.gov/pubmed/28700593
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0179625
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