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Combined single-cell quantitation of host and SIV genes and proteins ex vivo reveals host-pathogen interactions in individual cells

CD4 T cells harboring HIV-1/SIV represent a formidable hurdle to eradicating infection, and yet their detailed phenotype remains unknown. Here we integrate two single-cell technologies, flow cytometry and highly multiplexed quantitative RT-PCR, to characterize SIV-infected CD4 T cells directly ex vi...

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Détails bibliographiques
Publié dans:PLoS Pathog
Auteurs principaux: Bolton, Diane L., McGinnis, Kathleen, Finak, Greg, Chattopadhyay, Pratip, Gottardo, Raphael, Roederer, Mario
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2017
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5507340/
https://ncbi.nlm.nih.gov/pubmed/28654687
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.ppat.1006445
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