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Sandwich probes: two simultaneous reactions for templated nucleic acid detection

Fluorescence-quenched nucleic acid probes with reactive moieties at both the 5′ and 3′ ends are synthesized and tested for reaction with two adjacent nucleophile-containing DNAs. These probes improve signal to background over singly reactive probes and can discriminate single nucleotide polymorphism...

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Publicat a:Chem Commun (Camb)
Autors principals: Kleinbaum, Daniel J., Kool, Eric T.
Format: Artigo
Idioma:Inglês
Publicat: 2010
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5504956/
https://ncbi.nlm.nih.gov/pubmed/20927470
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c0cc01968b
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