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Double Displacement: an Improved Bioorthogonal Reaction Strategy for Templated Nucleic Acid Detection
Quenched autoligation probes have been employed previously in a target-templated nonenzymatic ligation strategy for detecting nucleic acids in cells by fluorescence. A common source of background signal in such probes is undesired reaction with water and other cellular nucleophiles. Here we describe...
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| Main Authors: | , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2010
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2891565/ https://ncbi.nlm.nih.gov/pubmed/20509625 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bc100165h |
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