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Double Displacement: an Improved Bioorthogonal Reaction Strategy for Templated Nucleic Acid Detection

Quenched autoligation probes have been employed previously in a target-templated nonenzymatic ligation strategy for detecting nucleic acids in cells by fluorescence. A common source of background signal in such probes is undesired reaction with water and other cellular nucleophiles. Here we describe...

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Библиографические подробности
Главные авторы: Kleinbaum, Daniel J., Miller, Gregory P., Kool, Eric T.
Формат: Artigo
Язык:Inglês
Опубликовано: 2010
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2891565/
https://ncbi.nlm.nih.gov/pubmed/20509625
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bc100165h
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