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A phased strategy to differentiate human CD14(+) monocytes into classically-activated, alternatively activated macrophages and dendritic cells

There are currently several in vitro strategies to differentiate human CD14(+) monocytes isolated from peripheral blood mononuclear cells into M1 or M2 macrophage cell types. Thereafter, each cell type is then verified using flow cytometric analysis of cell surface markers. Human CD14(+) monocytes h...

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Bibliografische gegevens
Gepubliceerd in:Biotechniques
Hoofdauteurs: Zarif, Jelani C., Hernandez, James R., Verdone, James E., Campbell, Scott P., Drake, Charles G., Pienta, Kenneth J.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2016
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5504907/
https://ncbi.nlm.nih.gov/pubmed/27401672
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114435
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