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A phased strategy to differentiate human CD14(+) monocytes into classically-activated, alternatively activated macrophages and dendritic cells

There are currently several in vitro strategies to differentiate human CD14(+) monocytes isolated from peripheral blood mononuclear cells into M1 or M2 macrophage cell types. Thereafter, each cell type is then verified using flow cytometric analysis of cell surface markers. Human CD14(+) monocytes h...

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Detalhes bibliográficos
Publicado no:Biotechniques
Main Authors: Zarif, Jelani C., Hernandez, James R., Verdone, James E., Campbell, Scott P., Drake, Charles G., Pienta, Kenneth J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5504907/
https://ncbi.nlm.nih.gov/pubmed/27401672
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114435
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