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A lentivirus-free inducible CRISPR-Cas9 system for efficient targeting of human genes
CRISPR-Cas9 is a cutting-edge tool for modifying genomes. The efficacy with which Cas9 recognizes its target has revolutionized the engineering of knockouts. However this efficacy complicates the knocking out of important genes in cultured cells. Unedited cells holding a survival advantage within an...
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| Publicado no: | Anal Biochem |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5501077/ https://ncbi.nlm.nih.gov/pubmed/28477963 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2017.05.001 |
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