Monoclonal Antibodies to Recombinant Fag e 3 Buckwheat Allergen and Development of a Two-site ELISA for Its Quantification

PURPOSE: Buckwheat is a major cause of anaphylaxis, and Fag e 3 is the key major allergen in buckwheat. However, an immunoassay system for the quantification of Fag e 3 has yet to be developed. METHODS: We developed a 2-site enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies (mAbs...

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Detalhes bibliográficos
Publicado no:Allergy Asthma Immunol Res
Main Authors: Jeong, Kyoung Yong, Park, Kyung Hee, Lee, Jae-Hyun, Park, Jung-Won
Formato: Artigo
Idioma:Inglês
Publicado em: The Korean Academy of Asthma, Allergy and Clinical Immunology; The Korean Academy of Pediatric Allergy and Respiratory Disease 2017
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Original Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5500696/
https://ncbi.nlm.nih.gov/pubmed/28677355
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4168/aair.2017.9.5.417
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