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Flexible CRISPR library construction using parallel oligonucleotide retrieval
CRISPR/Cas9-based gene knockout libraries have emerged as a powerful tool for functional screens. We present here a set of pre-designed human and mouse sgRNA sequences that are optimized for both high on-target potency and low off-target effect. To maximize the chance of target gene inactivation, sg...
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| Pubblicato in: | Nucleic Acids Res |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
2017
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5499874/ https://ncbi.nlm.nih.gov/pubmed/28334828 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx181 |
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