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Increasing quality, throughput and speed of sample preparation for strand-specific messenger RNA sequencing

BACKGROUND: RNA-Sequencing (RNA-seq) is now commonly used to reveal quantitative spatiotemporal snapshots of the transcriptome, the structures of transcripts (splice variants and fusions) and landscapes of expressed mutations. However, standard approaches for library construction typically require r...

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Dettagli Bibliografici
Pubblicato in:BMC Genomics
Autori principali: Haile, Simon, Corbett, Richard D., MacLeod, Tina, Bilobram, Steve, Smailus, Duane, Tsao, Philip, Kirk, Heather, McDonald, Helen, Pandoh, Pawan, Bala, Miruna, Hirst, Martin, Miller, Diane, Moore, Richard A., Mungall, Andrew J., Schein, Jacquie, Coope, Robin J., Ma, Yussanne, Zhao, Yongjun, Holt, Rob A., Jones, Steven J., Marra, Marco A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2017
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5499059/
https://ncbi.nlm.nih.gov/pubmed/28679365
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-017-3900-6
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