Expression of fully functional tetrameric human hemoglobin in Escherichia coli.
Synthetic genes encoding the human alpha- and beta-globin polypeptides have been expressed from a single operon in Escherichia coli. The alpha- and beta-globin polypeptides associate into soluble tetramers, incorporate heme, and accumulate to greater than 5% of the total cellular protein. Purified r...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54988/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2236062/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.21.8521 |
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