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Isoelectric point-based fractionation by HiRIEF coupled to LC-MS allows for in-depth quantitative analysis of the phosphoproteome

Protein phosphorylation is involved in the regulation of most eukaryotic cells functions and mass spectrometry-based analysis has made major contributions to our understanding of this regulation. However, low abundance of phosphorylated species presents a major challenge in achieving comprehensive p...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Panizza, Elena, Branca, Rui M. M., Oliviusson, Peter, Orre, Lukas M., Lehtiö, Janne
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5495806/
https://ncbi.nlm.nih.gov/pubmed/28674419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-04798-z
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