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Biochemical characterization and thermal inactivation of polyphenol oxidase from elephant foot yam (Amorphophallus paeoniifolius)

Polyphenol oxidase from Amorphophallus corm was purified (5.54 fold) by acetone precipitation and ion exchange chromatography. Electrophoretic-blot analysis revealed that the molecular weight of enzyme is 40 kDa. Enzyme preferred catechol as substrate and showed optimum activity at pH 6 and 35 °C wi...

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Detalhes bibliográficos
Publicado no:J Food Sci Technol
Main Authors: Singh, Anuradha, Wadhwa, Neeraj
Formato: Artigo
Idioma:Inglês
Publicado em: Springer India 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5495737/
https://ncbi.nlm.nih.gov/pubmed/28720966
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13197-017-2647-z
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