Discovery of active proteins directly from combinatorial randomized protein libraries without display, purification or sequencing: identification of novel zinc finger proteins

We have successfully linked protein library screening directly with the identification of active proteins, without the need for individual purification, display technologies or physical linkage between the protein and its encoding sequence. By using ‘MAX’ randomization we have rapidly constructed 60...

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Publié dans:	Nucleic Acids Res
Auteurs principaux:	Hughes, Marcus D., Zhang, Zhan-Ren, Sutherland, Andrew J., Santos, Albert F., Hine, Anna V.
Format:	Artigo
Langue:	Inglês
Publié:	Oxford University Press 2005
Sujets:	Methods Online
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC549430/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15722478/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gni031
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