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Discovery of active proteins directly from combinatorial randomized protein libraries without display, purification or sequencing: identification of novel zinc finger proteins

We have successfully linked protein library screening directly with the identification of active proteins, without the need for individual purification, display technologies or physical linkage between the protein and its encoding sequence. By using ‘MAX’ randomization we have rapidly constructed 60...

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Detalhes bibliográficos
Main Authors: Hughes, Marcus D., Zhang, Zhan-Ren, Sutherland, Andrew J., Santos, Albert F., Hine, Anna V.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC549430/
https://ncbi.nlm.nih.gov/pubmed/15722478
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni031
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