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Transposon-mediated generation of targeting vectors for the production of gene knockouts

Vectors used for gene targeting experiments usually consist of a selectable marker flanked by two regions of homology to the targeted gene. In a homologous recombination event, the selectable marker replaces an essential element of the target gene rendering it inactive. Other applications of gene ta...

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Hlavní autoři: Zhang, Chunfang, Kitsberg, Danny, Chy, Hun, Zhou, Qi, Morrison, John R.
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2005
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC549422/
https://ncbi.nlm.nih.gov/pubmed/15699181
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni014
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