Method for preparing DNA from feces in guanidine thiocyanate solution affects 16S rRNA-based profiling of human microbiota diversity

Metagenomic analysis based on the 16S rRNA gene is generally performed to examine the diversity and abundance of commensal bacteria in feces, which is now recognized to be associated with human health and diseases. Guanidine thiocyanate (GuSCN) solution is used as a less onerous way compared with a...

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Udgivet i:Sci Rep
Main Authors: Hosomi, Koji, Ohno, Harumi, Murakami, Haruka, Natsume-Kitatani, Yayoi, Tanisawa, Kumpei, Hirata, Soichiro, Suzuki, Hidehiko, Nagatake, Takahiro, Nishino, Tomomi, Mizuguchi, Kenji, Miyachi, Motohiko, Kunisawa, Jun
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2017
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5489508/
https://ncbi.nlm.nih.gov/pubmed/28659635
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-04511-0
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