Protein composition of mammalian spliceosomes assembled in vitro.
This paper reports an analysis of the protein composition of highly purified mammalian spliceosomes isolated by a two-step large-scale affinity chromatography procedure. Splicing complexes were assembled in vitro on biotinylated pre-mRNA, fractionated by gel filtration, and then affinity-purified by...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54886/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2146679/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.20.8031 |
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