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NMR screening and crystal quality of bacterially expressed prokaryotic and eukaryotic proteins in a structural genomics pipeline

In the Joint Center for Structural Genomics, one-dimensional (1D) (1)H NMR spectroscopy is routinely used to characterize the folded state of protein targets and, thus, serves to guide subsequent crystallization efforts and to identify proteins for NMR structure determination. Here, we describe 1D (...

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Hlavní autoři: Page, Rebecca, Peti, Wolfgang, Wilson, Ian A., Stevens, Raymond C., Wüthrich, Kurt
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2005
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC548552/
https://ncbi.nlm.nih.gov/pubmed/15677718
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0408490102
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