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NMR screening and crystal quality of bacterially expressed prokaryotic and eukaryotic proteins in a structural genomics pipeline

In the Joint Center for Structural Genomics, one-dimensional (1D) (1)H NMR spectroscopy is routinely used to characterize the folded state of protein targets and, thus, serves to guide subsequent crystallization efforts and to identify proteins for NMR structure determination. Here, we describe 1D (...

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Bibliografiske detaljer
Main Authors: Page, Rebecca, Peti, Wolfgang, Wilson, Ian A., Stevens, Raymond C., Wüthrich, Kurt
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2005
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC548552/
https://ncbi.nlm.nih.gov/pubmed/15677718
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0408490102
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