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A Simple Phosphate-Buffered-Saline-Based Extraction Method Improves Specificity of HIV Viral Load Monitoring Using Dried Blood Spots

Although Roche COBAS Ampliprep/COBAS TaqMan (CAP/CTM) systems are widely used in sub-Saharan Africa for early infant diagnosis of HIV from dried blood spots (DBS), viral load monitoring with this system is not practical due to nonspecific extraction of both cell-free and cell-associated viral nuclei...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:J Clin Microbiol
Hauptverfasser: Makadzange, A. Tariro, Boyd, F. Kathryn, Chimukangara, Benjamin, Masimirembwa, Collen, Katzenstein, David, Ndhlovu, Chiratidzo E.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2017
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5483919/
https://ncbi.nlm.nih.gov/pubmed/28468852
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00176-17
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