Evaluating flow cytometer performance with weighted quadratic least squares analysis of LED and multi-level bead data

We developed a fully automated procedure for analyzing data from LED pulses and multi-level bead sets to evaluate backgrounds and photoelectron scales of cytometer fluorescence channels. The method improves on previous formulations by fitting a full quadratic model with appropriate weighting and by...

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Bibliografische gegevens
Gepubliceerd in:Cytometry A
Hoofdauteurs: Parks, David R., Khettabi, Faysal El, Chase, Eric, Hoffman, Robert A., Perfetto, Stephen P., Spidlen, Josef, Wood, James C.S., Moore, Wayne A., Brinkman, Ryan R.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2017
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5483398/
https://ncbi.nlm.nih.gov/pubmed/28160404
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cyto.a.23052
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