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Evaluating flow cytometer performance with weighted quadratic least squares analysis of LED and multi-level bead data
We developed a fully automated procedure for analyzing data from LED pulses and multi-level bead sets to evaluate backgrounds and photoelectron scales of cytometer fluorescence channels. The method improves on previous formulations by fitting a full quadratic model with appropriate weighting and by...
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| Publicado no: | Cytometry A |
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| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5483398/ https://ncbi.nlm.nih.gov/pubmed/28160404 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cyto.a.23052 |
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