Overexpression and characterization of two types of nitrile hydratases from Rhodococcus rhodochrous J1

Nitrile hydratase (NHase) from Rhodococcus rhodochrous J1 is widely used for industrial production of acrylamide and nicotinamide. However, the two types of NHases (L-NHase and H-NHase) from R. rhodochrous J1 were only slightly expressed in E. coli by routine methods, which limits the comprehensive...

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Udgivet i:PLoS One
Main Authors: Lan, Yao, Zhang, Xiaohuan, Liu, Zhongmei, Zhou, Li, Shen, Ruihua, Zhong, Xianping, Cui, Wenjing, Zhou, Zhemin
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2017
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5482477/
https://ncbi.nlm.nih.gov/pubmed/28644864
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0179833
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