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Purification and characterization of nitrile hydratase of mutant 4D of Rhodococcus rhodochrous PA-34

Nitrile hydratase (NHase; E.C. 4.2.1.84) has been purified and characterized using ammonium sulfate precipitation, ion exchange chromatography and gel filtration chromatography from the mutant 4D of Rhodococcus rhodochrous PA-34. The SDS-PAGE and MALDI-TOF analysis of the purified enzyme revealed th...

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Bibliografische gegevens
Hoofdauteurs: Pratush, Amit, Seth, Amit, Bhalla, Tek Chand
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Springer Berlin Heidelberg 2012
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3597139/
https://ncbi.nlm.nih.gov/pubmed/28324571
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-012-0081-5
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