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Ten-minute protein purification and surface tethering for continuous-flow biocatalysis
Nature applies enzymatic assembly lines to synthesize bioactive compounds. Inspired by such capabilities, we have developed a facile method for spatially segregating attached enzymes in a continuous-flow, vortex fluidic device (VFD). Fused His(n)-tags at the protein termini allow rapid bioconjugatio...
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| 出版年: | Angew Chem Int Ed Engl |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2017
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5480406/ https://ncbi.nlm.nih.gov/pubmed/28133915 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201610821 |
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