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Ten-minute protein purification and surface tethering for continuous-flow biocatalysis

Nature applies enzymatic assembly lines to synthesize bioactive compounds. Inspired by such capabilities, we have developed a facile method for spatially segregating attached enzymes in a continuous-flow, vortex fluidic device (VFD). Fused His(n)-tags at the protein termini allow rapid bioconjugatio...

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發表在:Angew Chem Int Ed Engl
Main Authors: Britton, Joshua, Dyer, Rebekah P., Majumdar, Sudipta, Raston, Colin L., Weiss, Gregory A.
格式: Artigo
語言:Inglês
出版: 2017
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5480406/
https://ncbi.nlm.nih.gov/pubmed/28133915
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201610821
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